Vimentin anticorps

N° du produit ABIN204914

L’anticorps Souris Monoclonal anti-Vimentin a été validé pour WB, IHC, FACS, IHC (p), ICC et IHC (fro). Il convient pour détecter Vimentin dans des échantillons de Humain, Rat et Poulet.

Aperçu rapide pour Vimentin anticorps (ABIN204914)

Antigène: Vimentin (VIM)

Reactivité: Humain, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Vimentin anticorps

Specificité: Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.

Purification: Protein G purified

Immunogène: Vimentin.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)

Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer,  pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis